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Fig. 1 Establishment of a Socs3a KO line. (A) Schematic diagram of the Socs3a protein indicating constituent domains (KIR, kinase inhibitory region; ESS, extended SH2 sub-domain; SH2, Src homology 2 domain; SOCS box, suppressor of cytokine signaling box) as well as socs3a gene showing the intron (thin line) and exons (boxes) and sequences coding each domain and targeted with the sgRNAs. (B) Sequence traces of representative homozygous wildtype (WT, socs3awt/wt) and knockout (KO, socs3ako/ko) fish at the genomic region targeted by the sgRNA showing nucleotides and encoded amino acids. Nucleotide sequences that were deleted in the mutant are boxed purple, with de novo protein sequences shown in red. (C) Schematic of Socs3a protein forms in WT and KO fish.