ZFIN Image: Schloss et al., 2025, Fig 4

Image

Figure Caption

Fig 4 cdh16 is expressed in the corpuscles of Stannius (CS) during embryonic and larval development.

(A–G) Whole-mount in situ hybridization chain reaction (HCR) using probes against sequences contained within the cdh16^co120 large deletion (A), or full cdh16 probe set (B–G). Maximum projections of confocal stacks show the whole larval zebrafish (A), pronephros (B), and corpuscles (C–G). (A) At 120 hpf, cdh16 is expressed in the corpuscles of Stannius (red box). Background signal is observed elsewhere, including in the eyes and head. (B)cdh16 puncta are enriched in distal pronephros where the CS will be extruded. (C–F)cdh16 signal is present in the CS and kidney from 48 hpf to 120 hpf. (G) By 144 hpf cdh16 signal is present in the CS but is no longer detectable in the kidney. Shown are representative images, n = 5 larvae were imaged for whole-body and per time point.

Acknowledgments

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