|
Fig. 4 Two-photon laser ablations of tph2+ neurons in pineal and pretectum did not influence the emergence from propofol. A, Behavioral experiments diagram. B, The representative images of two-photon laser ablation on pineal and pretectum. C, The spontaneous movement trajectory of the control group, mock ablation group, and pineal + pretectum ablation group during 10 min. D, Distance moved during 1 h and the statistical analysis (control vs mock ablation vs pineal + preT ablation; 349.8 ± 18.0 mm vs 330.1 ± 17.5 mm vs 336.5 ± 17.7 mm; p > 0.05; unpaired Student's t test). E,F, Two-photon laser ablations of pineal and pretectum did not influence the emergence time from propofol anesthesia, detected by both recovery to mechanical stimulation (E, control, 11.3 ± 1.0 min; mock ablation, 12.3 ± 1.0 min; pineal + PreT ablation, 10.8 ± 1.0 min) and the recovery time of spontaneous movement (F, control, 71.3 ± 3.3 min; mock ablation, 74.5 ± 3.8 min; pineal + PreT ablation, 73.3 ± 3.5 min). p > 0.05; one-way ANOVA.