Figure 3

Figure 3 Low levels of Eif6 are enough for survival to adulthood, but only an absence of eif6 KO affects the levels of ribosomal proteins.

(A) Amino acid alignment of the 2 eif6 mutants (eif6^ms/ms and eif6^{del2aa/del2aa}) compared with the Eif6 WT sequence. (B) mRNA levels in all 3 eif6 mutants and WT zebrafish at 5 dpf. (C) Western blots showing Eif6, Sbds, and RP levels in the different eif6 mutants. (D) Western blot quantification. (E) Western blots showing Rpl23 and Rps3 in eif6^+/+, eif6^+/–, and eif6^–/– siblings at 5 dpf. (F) Western blot quantification relative to actin. (G) Western blotting showing Rpl23 and Rps3 expression in eif6^+/+, eif6^{del2aa/del2aa}, and eif6^ms/ms zebrafish at 5 dpf. (H) Western blot quantification relative to actin. (I) Polysome profiles of eif6 WT and mutants at 5 dpf. *P < 0.05, **P < 0.01, and ***P < 0.001, by 1-way ANOVA. Data represent the mean ± SEM.