Fig. 3 SEMA5A-overexpressed zebrafish embryo model showed significant developmental abnormality. (A) Morphogenesis of live control (injection with GFP messenger RNA [mRNA]) and SEMA5A-overexpressed (injection with SEMA5A mRNA) embryos at 3dpf; SEMA5A morphants at approximately 3dpf are displayed according to increasing levels of severity of axial development (classified 1–3, respectively). Dose-dependent effect of SEMA5A overexpression on zebrafish embryo malformation (B), hatching rates (C), body length (D), and survival rates (E) at 3 dpf. Values significantly different from the control are indicated by asterisks (*p < 0.05, **p < 0.01, and ***p < 0.001).
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