ZFIN Image: Li et al., 2025, Figure 1

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Figure 1

The experimental workflow chart. Zebrafish at 1 day (s) post fertilization (dpf) were exposed to MPTP and different fractions of EUOL extract. The 30% EF was identified to have the best anti-PD activity by evaluating the development of dopaminergic neurons and neuronal vasculature in the brain at 4 dpf. At 6 dpf, we monitored zebrafish behavior and tested the expression of autophagy-related genes. Then, we verified the anti-PD activity of 30% EF using the zebrafish and cellular PD model by detecting the expression of α-syn and LC3B. Finally, we investigated the underlying neuroprotective mechanism by locomotion assays, molecular docking simulation, and SPR.

Acknowledgments

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