ZFIN Image: Doan et al., 2025, Figure 1.

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Figure Caption

Figure 1.

Age-dependent myelin maintenance defects are present in dock1 MUTs. (A–C) TEM of cross-sections of ZMBs from 4-mo-old WT dock1^+/+, HET dock1^stl145/+, and homozygous dock1^{stl145/stl145} MUT zebrafish. (D–F) TEM micrographs of ZMBs from 12-mo-old WT dock1^+/+, HET dock1^stl145/+, and homozygous dock1^{stl145/stl145} MUT zebrafish, with homozygous MUTs exhibiting myelin outfoldings (red arrow). (G and H) Higher-magnification TEM micrographs of ZMBs from 12-mo-old homozygous dock1^{stl145/stl145} MUTs showing abnormally myelinated axons (red arrows), features rarely seen in WT dock1^+/+ or HET dock1^stl145/+ animals. (I) Quantification of the percent of axons with abnormal myelin profiles, observed by TEM, in WT dock1^+/+ versus homozygous dock1^{stl145/stl145} MUTs at 4 and 12 mo old, n = 10 (WT dock1^+/+ 4 mo old), 10 (dock1^{stl145/stl145} MUT 4 mo old), 10 (WT dock1^+/+ 12 mo old), and 10 (dock1^{stl145/stl145} MUT 12 mo old). Here, and in all figures, the X symbol in the graph denotes a data point corresponding to the representative image shown. (A–H) Scale bar = 1 µm. (I) Two-way ANOVA with Tukey’s multiple comparisons test. ***P < 0.001; ns, not significant.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Biol.