Fig 5

Fig 5 Induction of DN-Jun inhibits endogenous jun expression during larval optic nerve regeneration.

A. Experimental workflow and RT-qPCR results for evaluation of jun expression during optic nerve regeneration in Tg(isl2b:GFP) fish. At 5 dpf, transections were performed on the left optic nerve. Retinas were dissected and RNA was extracted at 0 hpt, 6 hpt, 24 hpt, 48 hpt, and 72 hpt to use for RT-qPCR. B. Experimental workflow and RT-qPCR results for evaluation of jun expression during optic nerve regeneration in Tg(isl2b:GFP) x Tg(mke15Tg) DN-Jun(+) progeny. Larvae were heat shocked at 4 and 5 dpf, after which they were screened, and transections were performed on the left optic nerve. Retinas were dissected and RNA was extracted at 0 hpt, 6 hpt, 24 hpt, 48 hpt, and 72 hpt to use for RT-qPCR. Each time point for each replicate represents n =  30 pooled retinas. Two-way ANOVA with Bonferroni Multiple Comparisons was performed to determine statistical significance. ** =  p < 0.01; **** =  p < 0.0001.