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Figure Caption
Fig. 1 CRISPR-Cas9 mediated gene KO of nr4a2a and nr4a2b. (a) Schematic representation of sgRNAs targeting 5′ and 3’ UTR regions of nr4a2a. PCR primers used for genotyping are shown in blue. (b) Sequencing and genotyping confirmation of nr4a2a mutagenesis. (c) Schematic representation of sgRNAs targeting 5′ and 3′ UTR regions of nr4a2b. (d) Confirmation of nr4a2b mutagenesis through genotyping and sequencing. For (b, d), lanes from the same gel have been combined for presentation purposes. A photograph of the original gel is provided as Figure S1.
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Full text @ J. Neurochem.