Fig 4

Fig 4 Early induction of DN-Jun results in developmental defects associated with jun expression.

A. Graphical timeline of experimental workflow. Each DN-Jun strain (mke14Tg, mke15Tg, and mke17Tg) was crossed with Tg(isl2b:GFP); and progeny were heat shocked as described. Fish that were positive for the DN-Jun protein are referred to as DN-Jun(+), siblings that did not express DN-Jun are referred to as DN-Jun(-). (B–C’) Representative brightfield images following experimental procedure outlined in A. B.Tg(isl2b:GFP) X Tg(mke17Tg) DN-Jun(-) fish, 72 hpf, lateral view. C. Tg(isl2b:GFP) X Tg(mke17Tg) DN-Jun(+) fish, 72 hpf, lateral view. B’. Lower jaw and ceratobranchial arches outlined in green as region of interest, and yellow dotted line showing jaw extension in DN-Jun(-) fish. C’. Red outline of lower jaw and ceratobranchial arches as region of interest and yellow dotted line marking jaw extension past the central point of the eye in DN-Jun(+) fish. m =  Meckel’s cartilage, pq =  palatoquadrate cartilage, cb =  ceratobranchial arches. Scale bar =  100 µm.