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Fig. 7 Cebpb-Nos2a acts downstream of Gcsf signaling. (A) Rescue assay of cebpb RNA. (B) Real-time quantitative polymerase chain reaction results for inosa in Rnf111 mutants compared with wild-type embryos at 36 hpf, 2 dpf and 3 dpf. The data are presented as mean ± standard deviation (SD) with **P<0.01, NS: no significant difference. (C) DAF-FM assay showed the decreased production of nitric oxide (NO) morphants at 2 dpf and 3 dpf. (D) Statistics of NO fluorescence intensity. The data are presented as mean ± SD with **P<0.01, ***P<0.001, NS: no significant difference. (E) A rescue effect of the NO agonist S nitroso N-acetylpenicillamine (SNAP) on cmyb expression was observed in Rnf111 mutants. Red arrows indicate cmyb-positive hematopoietic stem and progenitor cells in caudal hematopoietic tissue. All experiments were independently replicated at least three times. dpf: days post-fertilization; MU: Rnf111 mutants with deletion of 4 base pair nucleotides; hpf: hours post-fertilization; WT: wild-type; MO rnf111: rnf111 morphants; DAF-FM: 4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate.