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Fig. 3 Schematic representation and rescue assay of the four Rnf111 constructs. (A) Schematic diagram of the Rnf111 wild-type, Rnf111 RING mutant, Rnf111 SIM mutant and -4 base pair (bp) mutant proteins. (B) Whole-mount in situ hybridization analysis of the rescue efficiency of Rnf111 wild-type, Rnf111 RING mutant, Rnf111 SIM mutant and Rnf111-4 bp mutant RNA. Red arrows indicate cmyb-positive hematopoietic stem and progenitor cells. All experiments were independently replicated at least three times. WT: wild-type; RING MU: Rnf111 RING mutant with the RING domain mutated; SIM MU: Rnf111 SIM mutant with three SUMO-interacting motifs all mutated; SIM: SUMO-interacting motifs; -4bp MU: Rnf111-4 bp mutant with deletion of 4 bp nucleotides; dpf: days post-fertilization; MU: Rnf111 mutants with deletion of 4 bp nucleotides.