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Fig. 2 Impairment of definitive hematopoiesis in Rnf111 mutants. (A) Whole-mount in situ hybridization (WISH) analysis of cmyb expression from 36 hpf to 8 dpf. (B) WISH assay of runx1 in mutants at 36 hpf. (C) WISH analysis of key hematopoietic markers and Sudan black analysis. All experiments were independently replicated at least three times. MU: Rnf111 mutants with deletion of 4 base pair nucleotides; hpf: hours post-fertilization; dpf: days post-fertilization.
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