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Fig. 1 Generation of the Rnf111 mutant line. (A) The Cas9 target site was in the fifth exon; 4 base pair (bp) nucleotides were deleted. (B) The wild-type and mutant sequencing peak maps. (C) Schematic representation of wild-type and mutant Rnf111 proteins. (D) Western blot of HA-tagged wild-type and mutant Rnf111 proteins expressed in HEK293T cells. (E) CAGA12-luciferase transcriptional reporter assay of wild-type and mutant Rnf111. The data are presented as mean ± standard deviation with **P<0.01. WT: wild-type; MU: Rnf111 mutants with deletion of 4 bp; SIM: SUMO-interacting motifs.
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