Figure 4

Figure 4

Loss of mtmr5 results in microcephaly and an overall body size reduction from as early as 10 dpf. (A, top) A representative image of a WT 7 dpf zebrafish with lines delineating the measured gross anatomy. This system is used to measure all age groups (7 dpf, 10 dpf, 14 dpf and 2 months). (A, middle and bottom) At 7 dpf, there was no observed difference in (Ai) body length, (Aii) brain height or (Aiii) brain length between the siblings and KO. WT n = 20, heterozygous (Het) = 36, and KO n = 17. (B) Microcephaly (i.e. reduced absolute brain size) starts to appear in the KO at 10 dpf, when (Bi) there was a significant reduction in body length, (Bii) brain height and (Biii) brain length in the KO. WT n = 16, Het n = 40, KO n = 16. (C and D) Similarly, at 14 dpf (C) and at 2 months (D), there was a significant decrease in body length (Ci and Di), brain height (Cii and Dii) and brain length (Ciii and Diii) in KO compared to WT siblings. 14 dpf: WT n = 27, Het n = 45, KO n = 18. 2 months: WT n = 5, Het n = 8, KO n = 10. All statistical analyses include at least three independent experiments. Each dot on the graphs represents one zebrafish, at least n = 5 zebrafish were used per group per experiment. Quantitative data are mean±SEM, normalized to the average of WT and presented as ratios. One-way ANOVA was done for all measurements. *P < 0.05; **P < 0.01; ****P < 0.0001; ns, not significant. Scale bars: 500 μm (7, 10 and 14 dpf images) or 20 mm (adult images).