Fig. 5

Fig. 5 Nhsl1b localises at cell-cell contacts and at the tip of actin-rich protrusions.

Nhsl1b-mNeongreen and Lifeact-mCherry expressing mesodermal cells plated on a coverslip (A–C) or in vivo, transplanted in a non-labelled host embryo (D, E). A Nhsl1b localises at the very edge of the lamellipodium (arrowhead), where it stands during lamellipodium progression, as revealed by the kymograph (performed over the region delineated by the dashed yellow box). B, C In cultured cell clusters, Nhsl1b localises at cell-cell contacts (arrow) and at the very tip of actin-rich protrusions (arrowhead). D, E Similar localisations are observed in mesodermal cells in vivo. C, E Quantification of the max-normalised intensity of Nhsl1b-mNeongreen and Lifeact-mCherry along the segments indicated in the inset. Scale bars: (A) 10 µm; 2 µm, (B) 20 µm; 10 µm, (D) 40 µm; 20 µm.