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FIGURE 1

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ZDB-IMAGE-250226-36
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Figures for Tanifuji et al., 2025
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Figure Caption

FIGURE 1

Angiotensin II (AngII) administration increased the dorsal aorta diameter in zebrafish embryos. (a) A lateral bright field image of Tg(kdrl:EGFP) control embryos at 5 days post‐fertilization (dpf). Scale bar: 1 mm. (b) A confocal microscopy image of the EGFP‐positive blood vessels of the boxed region in (a); m7 to m11 indicates myotomes 7–11, respectively. Scale bar: 100 μm. (c) Representative lateral dorsal aorta images of control or AngII (160 ng)‐injected Tg(kdrl:EGFP) embryos at 5 dpf. The lower panels are higher‐magnification images of the upper panels. Scale bars: 50 μm. (d) Quantification of the average diameter of the dorsal aorta. (e–h) Dorsal aorta diameter at myotomes 7–11 in control or AngII (40 ng, 80 ng, or 160 ng)‐injected Tg(kdrl:EGFP) embryos at 5 dpf. n = 15 (control), 15 (40 ng), 13 (80 ng), and 18 (160 ng). (i–k) Dorsal aorta diameter at myotomes 7–11 in control or AngII (160 ng)‐injected Tg(kdrl:EGFP) embryos at 7 dpf (i), 10 dpf (j), and 14 dpf (k). (l) Quantification of the average diameter of the five serial segments of the dorsal aorta. n = 14 (control, white), and n = 14 (AngII 160 ng, gray). NS indicates not significant. Dotted lines in (b) and (c) indicate the diameter of the dorsal aortae. DA, dorsal aorta; DLAV, dorsal longitudinal anastomotic vessel; ISV, intersegmental vessel; PCV, posterior cardinal vein.

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