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Fig. 2 Human DLG5 over-expression prevent phenylhydrazine (PHZ)-induced zebrafish thrombosis. (a–l) Representative images of Tg (CD41: GFP) zebrafish embryos at 3-dpf treated with WT control (empty vector), human DLG5, WT control plus PHZ (2 μM) or human DLG5 plus PHZ (2 μM). Zebrafish at 2-dpf treated with 2-μM PHZ for 24 h visually showed severe thrombosis and platelet aggregation in the caudal vessels (c,g,k, red arrows) without observable toxicity. (c,d,g,h) Compared with PHZ treated group, human DLG5 over-expression prevent PHZ-induced zebrafish thrombosis. (m) Quantification of the average number of thrombocyte (GFP+) at caudal vein. Columns, mean; bars, SEM (n = 10; ANOVA; * P < 0.05). dpf, days post fertilization; PHZ, phenylhydrazine.