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Fig. EV3 p60-katanin and spastin transcripts are both expressed in developing spinal motor neurons. (A) Whole-mount in situ hybridisation with p60-katanin sense (upper panel) and antisense (bottom panel) riboprobes at 18 somites and 24 h post-fertilisation (hpf). Lateral views of the embryos, anterior to the left. P60-katanin is highly enriched in the developing nervous system at both 18 somites and 24 hpf, two stages at which the axons of primary (pMN) and secondary (sMN) motor neurons exit the spinal cord to navigate towards their muscle targets. Scale bars: 200 µm. (B) In toto hybridisation chain reaction (HCR) on 72-hpf Tg(Hb9:GFP) larvae using zebrafish spastin and katna1 probes. Lateral view of the trunk, anterior to the left. Right panels are higher magnifications of boxed region of the corresponding left panel. Arrows point at spinal motor neurons co-expressing katna1 and spastin transcripts. Scale bars: 20 µm. Source data are available online for this figure.