|
Fig. 1 Cigarette Smoke Extract Exposure Inhibits Craniofacial Development in Zebrafish Larvae. (A) Ventral views of Alcian-stained larval craniofacial cartilage exposed to cigarette smoke extract (CSE) for 7 dpf. Bar charts present quantitative data for various metrics: (B) ceratohyal (CH) Length; (C) palatoquadrate (PQ) Length; (D) CH-PQ Angle; (E) CH-CH Angle; (F) Meckel’s-Meckel’s Angle; (G) PQ-Meckel’s Angle in response to CSE exposure. Data represent the average of 3 independent experiments (10 larvae/replicate). * p < 0.05, ** p < 0.01, *** p < 0.001. (H) Cartilage sections of zebrafish larvae exposed to CSE were also examined by Hematoxylin and Eosin (HE) staining. Scale bars represent 50 μm (lower) and 100 μm (upper).