Figure 2

Figure 2

MB cell lines express DHODH and are sensitive to pharmacological treatment with BRQ. (A) DHODH expression in paediatric tumour cell lines of the CCLE dataset, grouped by primary disease. (B) DHODH and MYC expression in medulloblastoma cell lines of the CCLE dataset. (C,D) Dose-response curves for cell viability (assessed by WST1 assay) after 72 h of BRQ treatment in a panel of seven medulloblastoma cell lines. shh: DAOY, Group 3: D425, D458, MB-LU-181, Med8A; and Group 4: CHLA-01-MED, CHLA-01R-MED. Cell lines marked as primary * or metastatic #. (E) Examples of compromised tumour aggregate formation of the suspension cell lines (E) D425 and D458 after pharmacological treatment with BRQ IC50 for 72 h. Cells were images at 10× magnification. Scale bars, 100 μm.