ZFIN Image: Ten Martin et al., 2024, Figure 9

Image

Figure Caption

Figure 9 TTLL11, but not TTLL6, rescues the axonal swelling phenotype caused by spastin haploinsufficiency in mammalian cortical neurons.

(A) Immunolabelling of β-III tubulin and GFP on DIV9 Sp + /+, Sp + /- and Sp-/- cortical neurons transduced or not at DIV2 with lentiviruses encoding GFP-tagged wild-type or catalytically dead variants (TTLL11d and TTLL6d) of mouse TTLL11 or TTLL6. Blue arrowheads and pink arrows, respectively, indicate non-swollen and swollen axons. Insets are higher magnifications of the distal part of the axons framed in the boxed region of each corresponding image. Arrowheads indicate growth cones. Scale bars: 20 μm. (B) Percentage of swollen axons. Blind quantifications were performed on at least 400 axons per condition pooled from two or three independent experiments. The “d” stands for catalytically dead variants of the TTLL enzymes. Chi2 test. P values are displayed on graphs. Source data are available online for this figure.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ EMBO J.