Figure 3

Figure 3

Depletion of Brcc3 in zebrafish embryos amplifies the up-regulatory response of p53 signaling to UV radiation. (A) Schematic diagram of UV radiation experiments for qRT-PCR analysis. (B) The relative mRNA levels of p53 and its target genes in WT and mutant embryos with or without UV radiation are accessed by qRT-PCR. Results are from three independent replicates. Values are represented as means ± s.d. ns, not significant; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 (one-way ANOVA followed by Tukey’s post hoc test). (C) Schematic diagram of UV radiation experimental design to access the p53 action after Brcc3 depletion. (D) Survival rate of brcc3 mutant embryos at 48 hpf in the p53^+/M214K or p53^M214K/M214K genetic background. The brcc3^{−2+3bp/−2+3bp}; p53^+/M214K and brcc3^{−2+3bp/−2+3bp}; p53^M214K/M214K were crossed, and the offsprings at 24 hpf were subjected to UV radiation for 30 s. The survival embryos at 48 hpf were subjected to genotyping analysis. Values are represented as means ± s.d. The total number of embryos is shown on each column.