Fig 4

Fig 4 Wnt signaling promotes 3’reg1 reporter expression and AC differentiation.

A-C) Confocal images of hearts from control, BIO-, and XAV-treated 3’reg1:GFP embryos stained for 3’reg1:GFP (green), Vmhc (red), Amhc (blue). D) The percentage of control and treated 3’reg1:GFP embryos with atrial, pan-cardiac, and lacking expression in their hearts. Control (n = 35); BIO (n = 32); XAV (n = 49). E) Normalized intensity of GFP expression in hearts of control and treated 3’reg1:GFP embryos. Control (n = 4); BIO (n = 6); XAV (n = 6). F-H) Confocal images of hearts from control, BIO-, and XAV-treated embryos stained for Nr2f1a (green), Mhc (pan-cardiac, red), Amhc (blue). I) The number of Nr2f1a⁺/Amhc⁺ cardiomyocytes with the hearts of control, BIO-, and XAV-treated embryos. Control (n = 12); BIO (n = 11); XAV (n = 11). J-L) Confocal images of hearts from control, BIO-, and XAV-treated myl7:DsRed2-NLS (myl7:DRN) embryos stained for DsRed-NLS (pan-cardiac nuclei—red) and Amhc (blue). M) The number of myl7:DsRed2-NLS⁺/Amhc⁺ cardiomyocytes (ACs) within the hearts of control, BIO-, and XAV-treated myl7:DsRed2-NLS embryos. Control (n = 9); BIO (n = 10); XAV (n = 8). Scale bars: 50 μm. Error bars in graphs indicate s.e.m. ** indicate P < 0.005, *** indicate P < 0.001. **** indicate P < 0.0001.