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Fig. 6 Effect of 4% HCD on bile acid signaling in zebrafish. A: Total green fluorescent protein fluorescence level in Tg(-1.7fabp6:GFP) reporter zebrafish with or without cyp7a1 mutation fed either 4% or the control diet. As fabp6 is an Fxr target, regulated by bile salts in zebrafish, this result suggests that our HCD could be used for modulating bile salt signaling in zebrafish. B: Representative brightfield and fluorescent images of WT and cyp7a1 mutant larvae fed either control diet or 4 % HCD. Two-way robust ANOVA and Games–Howell test were used for all samples. Scale = 0.2 mm. Fxr, farnesoid X receptor; HCD, high-cholesterol diet.