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Fig. 7 Slc4a7 mRNA compensation rescues phenotypes in slc4a7-deficient morphants. (A) Enlarged vertical and lateral views of larval eyeballs. Larvae injected with slc4a7 MO and full-length slc4a7 mRNA exhibit normal-sized eyeballs at 3 dpf. Scale bar = 100 mm. (B–D) Statistical analysis of axial length and ocular area. (E,F) VMR testing demonstrates significant recovery of both ON and OFF responses in slc4a7-deficient zebrafish compensated with mRNA compared to those without compensation. n = 20 in each group. Rescue experiments were repeated three times. Bar plots represent the mean ± s.e.m. Data were analyzed using one-way ANOVA followed by Tukey’s post hoc test, *** p < 0.001, **** p < 0.0001, indicate significant differences from the slc4a7 1.00 ng group. ns means no significance. The scattered circles of various colors in the bar chart denote individual values of zebrafish eye-related parameters for different groups.