|
Fig. 2 Inhibiting NMD results in a larger proportion of Δe3-gabrr2b mRNAs. (A) Representative gel from RT-PCR amplification around gabrr2b exon 3 from control larvae and those treated with 10, 25, or 50 μM of translation-inhibitor CHX for 24 h. (B) Average + SEM fraction of Δe3-gabrr2b transcripts as compared with +e3-gabrr2b transcripts as calculated from band intensities. n = 4–5 replicates per condition (shown as individual data points), with 40 larvae from a single clutch treated simultaneously before RT-PCR of each replicate. ***Control versus 50 μM CHX: p = 0.0006 (two-tailed unpaired t-test). CHX, cycloheximide; NMD, nonsense-mediated decay; SEM, standard error of the mean.