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Fig. 2 Schematic of embryo mounting in glass-bottom dish. A. Place a liquid drop of agarose/MBS on a glass-bottom dish. B. Immediately transfer the desired explant to the drop using a fire-polished pipette. C. Using forceps, gently orient the embryo until you adopt the proper position. A good marker of correct orientation is seeing a visible round notochord through the dissecting scope, as shown in the image (black arrow). D. Add MBS to cover the surface of the solidified agarose/MBS droplet. Using dip lenses may be necessary to image explant.
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