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Fig. 7 RetroMyelin regulates myelination in zebrafish Reduction of Mbp-promoter-driven EGFP expression upon genome editing of a small region of RetroMyelin sequences in a transgenic zebrafish reporter line (Tg(mbp:EGFP-CAAX) is shown. (A) Automated imaging of control and RetroMyelin gRNA injected zebrafish larva at 5 days postfertilization (5 dpf). Red boxes: locations of zoomed-in example regions in left panel. (B) No difference is found in larval morphological development, analyzed by eye area and body length. Adjusted p value after two-way ANOVA (Benjamini-Hochberg FDR post test), mean ± SEM, n = 52–60 (control), 19–23 (RetroMyelin gRNA) animals. (C) GFP fluorescence intensity along the dorsal and ventral tracts of the spinal cord was measured and plotted. ∗adjusted p < 0.05, ∗∗adjusted p < 0.01, two-way ANOVA (Benjamini-Hochberg FDR post test), mean ± SEM, n = 60 (control), 23 (RetroMyelin gRNA) animals. See also Figures S6 and S7.
Reprinted from Cell, 187, Ghosh, T., Almeida, R.G., Zhao, C., Mannioui, A., Martin, E., Fleet, A., Chen, C.Z., Assinck, P., Ellams, S., Gonzalez, G.A., Graham, S.C., Rowitch, D.H., Stott, K., Adams, I., Zalc, B., Goldman, N., Lyons, D.A., Franklin, R.J.M., A retroviral link to vertebrate myelination through retrotransposon-RNA-mediated control of myelin gene expression, 814830.e23814-830.e23, Copyright (2024) with permission from Elsevier. Full text @ Cell