Fig. 7

Fig. 7 Ascl1 regulates downstream effectors during dopaminergic differentiation. (A-H′) Whole-mount in situ hybridization for arxa (A-D′) and isl1 (E− H′) expression in 96 hpf embryos. Dorsal (A–H) and lateral views (A′-H′) of wildtype, single and double ascl1a and ascl1b mutant embryos. Anterior is to the left. Loss of Ascl1 function leads to a reduction of arxa expression (A-D′) in the telencephalon, prethalamus and preoptic region (arrowheads). The expression of isl1 (E-H′) is reduced in the telencephalon, prethalamus and hypothalamus (arrowheads) in double mutants compared to wildtype and single mutants. For numbers of analyzed embryos see Supplemental Table S2. Abbreviations: Hyp, hypothalamus; Po, preoptic region; Pth, prethalamus; Tel, telencephalon. Scale bars: 100 μm.