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Fig. 5 Five‐dpf‐old uppercut18 zebrafish are associated with altered transcriptional levels of SNARE complex members, protein interacting components, and components of the secretory pathway. (A) Transcriptional levels of stx18 are unaltered in uppercut18, but SNARE protein members including bnip1a, bnip1b, use1, sec22ba, and sec22bb are strongly upregulated, as well as the peripheral membrane protein components scfd1, zw10, and rint1. (B) Upregulated mRNA levels in uppercut18 zebrafish of all components investigated involved in early, mid, late anterograde, and retrograde secretory pathways (mia3, serpinh1, sec23a, sec24d, kdelr2a, copa, and arf1). Each data point represents the mean of 2 technical measurements from 5 biological replicates, with highlighted mean value (line). Two‐tailed unpaired t test; *p < 0.05, **p < 0.01, and ****p < 0.0001.