ZFIN Image: Cruz-Samperio et al., 2023, Fig. 5

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Figure Caption

Fig. 5 Characterization of AMBP-modified LNPs interaction with C2C12 myoblasts in flow. (a) End-point wide-field microscopy image of C2C12s cells in a flow channel after exposure with 1:2-LNPs or (b) 1:3-LNPs (250,000 cells per flow channel, approximately 1.5 × 1011 vesicles in 25 mL of DMEM) at a shear stress of 2 dyn for 15 min at 37 °C. GFP is shown in green, mCherry or Texas red is shown in red, and Hoechst 33342 is shown in blue. Scale bars: 50 μm. (c) Flow cytometry quantification of GFP (shown in green) and mCherry (shown in red) in C2C12 cells after the flow experiment (n = 3). Plot displays the mean ± SEM, and data points are shown. Each data point is the mean of fluorescence of 10,000 single-cell events. (d) DLS intensity data of 1-LNPs before and after exposure to 2 dyn stress shear showing that they maintain their size. LNP diagrams were created with Biorender.com.

Acknowledgments

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