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Fig. 3 Spatiotemporal expression of Ybx1 mRNA and protein in different tissues and ovarian follicles. (A) Semi-quantitative RT-PCR analysis of ybx1 and ef1a expression in different tissues. +, RT with M-MLV; −, RT without M-MLV. (B) Western blot detection of Ybx1 in different tissues. YB1N, anti-N-terminal antibody; YB1C, anti-C-terminal antibody; Bactin, β-actin. (C) Real-time PCR analysis of ybx1 expression in the follicles of different stages. Housekeeping gene, ef1a, was used as control. Different letters indicate statistical significance (n = 3) (D) Western blot detection of Ybx1 protein in different stages of follicles. (E) Immunohistochemical detection of Ybx1 in follicles. a, with antibodies YB1N or YB1C; b, antibodies neutralized with the antigenic peptides; c, pre-immune sera; d, no antibodies.