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Fig. 2 Structure of zebrafish Ybx1 and preparation of anti-Ybx1 antibodies. (A) Domain structure of human YB-1 and zebrafish Ybx1 proteins. AP, AP-rich N-terminal; CSD, cold shock domain; NLS, nuclear localization signal; CRS, cytosol localization signal; YB1N, anti-N-terminal antibody; YB1C, anti-C-terminal antibody. (B) Western blot testing for antibody specificity. YB1N could detect a major band at 48 kDa and a minor band at 36 kDa (asterisk) in PG follicle extract, whereas the YB1C could detect the major band only.
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