Fig. 1 Effects of acute hypoxia on apoptosis and metabolism in tilapia A: Survival rate of Nile tilapia during acute hypoxia (n=6 for three replicates). B: Proportion of early and late apoptosis in hepatocytes after exposure to normoxic (C) or hypoxic conditions for 12 h (12 h) in Nile tilapia primary hepatocytes. C: mRNA and protein expression of hif1α, hif2α, and hif3α; protein expression of Hif3α and Vegfa. D: Significantly changed metabolic KEGG pathways from RNA-seq. E: Differentially expressed genes (DEGs) related to lipid and glucose metabolism. F: Oil red staining. G: Triglyceride (TG) content in liver, free fatty acid (FFA) content in serum, mitochondrial fatty acid oxidation (FAO) in liver. H: Periodic acid-schiff (PAS) staining. I: Glycogen content in liver, glucose, and lactate in serum. *, **, and *** indicate significant differences (P<0.05), (P<0.01), and (P<0.001), respectively, between C and hypoxia 12 h groups.
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