ZFIN Image: Zhang et al., 2023, Fig. 3

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Figure Caption

Fig. 3 Wnt5b–Ror2–JNK signalling promotes the formation of long cytonemes.

a, Morphology phenotypes for indicated mutant lines. Ror2 mRNA (200 ng) was injected into the embryo as indicated. Arrowheads show abnormal tail morphology and heart oedema. Scale bar, 500 µm. b, Filopodia length in zebrafish embryos, split into indicated bins. Left to right: n = 367, 63, 113, 68, 312, 267, 41 and 59 measurements in 3 biological repeats. c, Zebrafish embryos were injected with indicated constructs and guide RNA (gRNA) and imaged at 6 hpf. Yellow arrows, spread of Wnt5b–GFP; blue arrows, Wnt5b–GFP on the plasma membrane. Scale bar, 10 µm. d, Wnt5b–GFP puncta spreading from producing clone, split into indicated bins. Left to right: n = 8 (Wnt5b–GFP), 5 (Wnt5b–GFP + Ror2) and 8 (ror1^cr/MZror2 + Wnt5b–GFP) embryos, 3 biological repeats. Two-way ANOVA with Dunnett’s multiple comparisons test. Box and whisker plots show median, and top and bottom quartile ranges, with whiskers extending to minimum and maximum values. P values are indicated.

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