ZFIN Image: Zhang et al., 2023, Fig. 5

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Fig. 5 Alterations of cytoneme-mediated dissemination of Wnt5b–Ror2 complexes affect convergence and extension in the zebrafish embryo.

a–i, Tg(−6gsc:EGFP–CAAX) zebrafish embryos were injected with control mRNA (a) or mRNA encoding Ror2 (b), Ror2–IRSp53^4K (c), Wnt5b–Ror2 (d), Wnt5b–Ror2–IRSp53^4K (e), IRSp53^4K (f), or CRISPR–ribonucleoprotein (RNP) complexes targeting ror1 and ror2 without (g) or with mRNA encoding Ror2 (h) or Ror2–IRSp53^4K (i), and imaged live at 10 hpf. Scale bar, 50 µm. The white line indicates the notochord width. White arrows mark cells that remain located lateral to the midline. j, The notochord width was measured in embryos treated as in a–i. Left to right: n = 20, 13, 12, 8, 12, 9, 6, 8 and 10 per embryo, 3 biological repeats. One-way ANOVA plus Tukey multiple comparisons test. Box and whisker plots show median, and top and bottom quartile ranges, with whiskers extending to minimum and maximum values. P values are indicated.

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