ZFIN Image: Li et al., 2020, Fig. 9

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Figure Caption

Fig. 9 Overexpression of fbxo3 inhibits K27-linked polyubiquitination of irf3 and irf7. (A) fbxo3 promoted the polyubiquitination of irf7 but not lysine 48 (K48)–linked polyubiquitination and lysine 63 (K63)–linked polyubiquitination. (B) fbxo3 promoted lysine 27 (K27)–linked polyubiquitination of irf7 but did not promote K6-, K11-, K29- and K33-linked polyubiquitination. (C) fbxo3 promoted K27-linked polyubiquitination of irf7. (D) fbxo3 promoted the polyubiquitination of irf3 but not K48-linked polyubiquitination and K63-linked polyubiquitination. (E) fbxo3 promoted the K27 polyubiquitination of irf3 but did not promote the K6-, K11-, K29-, and K33- inked polyubiquitination of irf3. (F) fbxo3 promoted K27-linked polyubiquitination of irf3. HEK 293T cells were transfected with HA-irf3 (6 μg) or HA-irf7 (6 μg), Myc-fbxo3 (4 μg), or empty vector (4 μg), together with His-ubiquitin or its mutants (4 μg). At 18 h posttransfection, the cells were treated with MG132 for 6 h. At 24 h posttransfection, the cells were lysed using guanidinium chloride, and purified with Ni2+-NTA agarose. IB, immunoblot; KO, K-only; KR, K is mutated to R.

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