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Fig. 7 Zebrafish fbxo3 inhibits RLR-mediated IFN induction and interacts with both irf3 and irf7. (A–D) Overexpression of fbxo3 suppressed rig-i–, mavs-, tbk1-, irf3-, and irf7-induced activation of ISRE (A), zebrafish IFN-φ1 promoter luciferase (B), EPC-IFN promoter luciferase (C) and zebrafish IFNφ3 promoter luciferase (D) reporter activity in EPC cells. (E and F) fbxo3 interacts with irf3 (E) and irf7 (F). HEK 293T cells seeded in 100-mm dishes were transfected with the indicated plasmids (5 μg each). After 24 h, total cell lysates were immunoprecipitated (IP) with anti-Myc agarose beads. Then the immunoprecipitates and cell lysates were detected with anti-Myc or anti-HA Ab, respectively. Data are presented as means ± SEM of three biological replicates. *p < 0.05, **p < 0.01. ns, no significance.