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Fig. 5 fbxo3-null zebrafish are more resistant to SVCV infection than WT zebrafish. (A) Representative images of fbxo3-null zebrafish larvae and their WT siblings (3 dpf) infected with or without SVCV for 18 h. Dead larvae exhibited lack of movement, absence of blood circulation and body degeneration. SVCV viruses (∼1 × 108 TCID50 per milliliter) was added into the water containing fbxo3-null larvae or the WT, and then the number of dead larvae was counted at different time points. (B) fbxo3-null zebrafish (n = 50 for each experiment) were more resistant to SVCV infection compared with the WT (n = 50 for each experiment) based on the survival ratio. (C–F) After challenge with SVCV for 12 h, induction of key antiviral genes including ifn1 (C), pkz (D), lta (E), and mxc (F) was more significant in fbxo3-null larvae compared with the WT larvae. (G–I) After challenge with SVCV for 24 h, the virus replication number indicated by the expression of the G (G), N (H), and P (I) genes of SVCV, was lower in the fbxo3-null ZFL compared with the WT ZFL infected with SVCV. Data are presented as means ± SEM of three biological replicates. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.