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Fig. 1 Zebrafish fbxo3 inhibits ifn activation induced by poly(I:C) stimulation and SVCV infection in EPC cells. (A–D) Overexpression of fbxo3 suppressed ISRE (A), zebrafish IFN-φ1 (B), IFN-φ3 (C), and EPC-IFN (D) reporter activity induced by poly(I:C) in EPC cells in a dose-dependent manner. EPC cells were transfected with each luciferase reporter (0.2 μg per well) together with HA empty vector or an increasing amount of the HA-fbxo3 vector (0.1 and 0.2 μg per well), respectively. After 24 h, the cells were transfected with poly(I:C) (1 μg/ml) for 24 h, and then luciferase reporter activity assays were performed. (E–H) Overexpression of fbxo3 suppressed ISRE (E), zebrafish IFN-φ1 (F), IFN-φ3 (G), and EPC-IFN (H) reporter activity in EPC cells induced by SVCV infection in a dose-dependent manner. EPC cells were transfected with each luciferase reporter (0.2 μg per well) together with HA empty vector or an increasing amount of the HA-fbxo3 vector (0.1 and 0.2 μg per well), respectively. After 24 h, the cells were infected with SVCV (∼1.0 × 108 TCID50 per milliliter) for 24 h and then conducted luciferase reporter activity assays. Data are presented as means ± SEM of three biological replicates. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.