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Figure 1—figure supplement 1. Validation of motion analysis based on image artefacts during two-photon imaging. (A) Motion indexes as calculated based on tail tracking (blue) and based on decreases in the correlation between individual frames and the reference frame used for motion alignment (orange) across the entire imaging experiment (65 min). (B) Same analysis as (A), for a different larva. (C) Cross-correlation plot comparing the two motion index vectors. Mean across six larvae, and line thickness = standard error.

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