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Fig. 1 Mn treatment affects hatching and induces heart, swim bladder, and skin alterations. Zebrafish eggs were treated with Mn at 0.5, 5, 50, 100, and 500 mg/L and analyzed under a light microscope. Only 500 mg/L of Mn showed a significant inhibition of hatching (A, I). The zebrafish larvae also showed alteration in the swim bladder after treatment with all Mn concentrations tested (B, D–G), whereas cardiac edema and skin aggregates were found only after treatment with Mn levels higher than 50 mg/L (C, F–H). The results are expressed as mean ± SEM. The groups were performed with n = 5, where ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05 versus control group. ns = nonsignificant. Statistical analysis was performed using one-way ANOVA followed by Tukey's post-test. Scale bar in C, H, and I = 500 μm. Arrowhead = cardiac edema; arrow = swim bladder; * = blood. ANOVA, analysis of variance; Mn, manganese; SEM, standard error of the mean. Color images are available online.