Fig 3

Fig 3 ZO1b-Beta is necessary for robust Connexin localization to electrical synapses.

A-J. Confocal images of Mauthner circuit neurons and stereotypical electrical synaptic contacts in 5 days post fertilization (dpf) zf206Et zebrafish larvae from wt (A, B), tjp1b/ZO1b-pan^-/- (C, D), tjp1b/ZO1b-alpha^-/- (E, F), tjp1b/ZO1b-beta^-/- (G, H), and tjp1b/ZO1b-gamma^-/- (I, J) animals. Animals are stained with anti-GFP (green), anti-Cx35.5 (cyan), anti-Cx34.1 (yellow), and anti-ZO1 (magenta). Scale bars = 2 μm. Boxed regions denote stereotyped location of electrical synapses and regions are enlarged in neighboring panels. Images of the Mauthner cell body and lateral dendrite in the hindbrain (A, C, E, G, I) are maximum intensity projections of ~10–20 μm. In A’, C’, E’, G’, and I’, images are maximum-intensity projections of ~3–6 μm and neighboring panels show the individual channels. Images of the sites of contact of M/CoLo processes in the spinal cord (B, D, F, H, J) are maximum-intensity projections of ~6–8 μm. In B’, D’, F’, H’ and J’, images are from a single 0.42 μm Z-plane and the white dashed circle denotes the location of the M/CoLo site of contact. Neighboring panels show individual channels. Anterior up.