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Fig. 3 Sample population clusters and the number of differentially expressed genes in glucose-exposed and FFA/TAG-injected embryos PCA plot displaying discrete and distinct population clusters between treatment groups. (A) Glucose exposed versus Control. (B) FFA/TAG injected versus Control. Venn Diagrams summarizing the intersection of genes between treatment groups. (C) Overall genes that were significantly differentially expressed between glucose-exposed versus control and FFA/TAG-injected versus control. (D) Downregulated genes differentially expressed in glucose-exposed versus control and FFA/TAG-injected versus control. (E) Upregulated genes differentially expressed in glucose-exposed versus control and FFA/TAG-injected versus control.