Fig. 3.

Fig. 3. Definitive macrophages are required for ovarian failure and sex reversal of bmp15 mutants.

(A to F) Live tissue pictures of adult gonads of bmp15 mutants when macrophages are (A) present, (B) and (C) completely ablated, (D) haploinsufficiency of, or lack of (E) primitive, or (F) definitive populations. Scale bar, 500 μm. St.III*, arrested stage III oocyte (increased size and apparent yolk). (G to L) Secondary sex traits of adult: (G) male body shape, (H) urogenital papilla (orange dashed line and arrowhead), and (I) lateral fin tubercles (blue arrowheads and box); (J) rounded female body shape, (K) distinct urogenital papilla (yellow dashed line and arrowhead), and (L) absence of tubercles (pink arrowheads and box). Scale bars, 1 mm. (M) Adult sex ratios of indicated genotypes. Female, pink; male, blue. Numbers indicate individuals examined. Statistical analysis: chi-square test with Bonferroni correction; P value comparisons to bmp15^+/−, *P ≤ 0.0125.