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Fig. 1 In vivo images at pre-and post-photoactivation. (A) In vivo cerebral angiography of vascular endothelial cells in transgenic zebrafish larvae [Tg (flk:gfp)] (GFP). (B) Evaluation of blood flow using the 560 nm laser fluorescence microscope (rhodamine). (C) Evaluation of blood flow in the endothelial lining by merging GFP and rhodamine. Blood flow in intracerebral vessels disappeared, and endothelial linings’ fluorescence intensity slightly decreased (D-F). GFP, green fluorescent protein.
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