ZFIN Image: Li et al., 2023, Fig. 2.

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Figure Caption

Fig. 2.

Csde1 regulates HSPC specification in an EC autonomous manner. (A) WISH analysis showing the expression of runx1 (arrowheads) in WT, csde1 mutants and csde1 mutants injected with hsp70: flag-hCSDE1^WT-EGFP or hsp70: flag-hCSDE1^DN-EGFP constructs at 36 hpf. (B) Quantification of the WISH data in A. (C) qPCR analysis of runx1 in WT, csde1 mutants and csde1 mutants injected with hsp70: flag-hCSDE1^WT-EGFP or hsp70: flag-hCSDE1^DN-EGFP constructs at 36 hpf. (D) WISH analysis showing the expression of runx1 (arrowheads) in WT, csde1 mutants and csde1 mutants injected with fli1a: flag-hCSDE1^WT-EGFP or fli1a: flag-hCSDE1^DN-EGFP constructs at 36 hpf. (E) Quantification of the WISH data in D. (F) qPCR analysis of runx1 in WT, csde1 mutants and csde1 mutants injected with fli1a: flag-hCSDE1^WT-EGFP or fli1a: flag-hCSDE1^DN-EGFP constructs at 36 hpf. Data are mean±s.d. **P<0.01, ***P<0.001, ****P<0.0001 (two-tailed unpaired Student's t-test). ns, not significant. n≥3 replicates. Numbers indicate the number of embryos with respective phenotype/total number of embryos analyzed in each experiment (A,D). Scale bars: 100 μm.

Acknowledgments

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