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Fig 6 Delayed epiboly progression and disruption of F-actin organization caused by KT09 extract treatment.
Embryos were treated with KT09 extract starting at 2hpf, while phalloidin and DAPI staining was performed at 80% epiboly (9hpf). (A-A”) A control embryo at 90% epiboly stage, with a dense marginal actin ring (white arrows). (B-B”) A representative embryo expose to KT09 extract shows epiboly retardation, and disruption of the EVL cortical F-actin, marginal actin ring and vegetal cortex F-actin. Scale bar: 100 μm.
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