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Fig. 6 Unique DE genes of HRAS-OE hearts compared with regenerating hearts. (A) Heatmap of log2-transformed normalized expression values for genes exclusively deregulated in cmlc2/GFP-HRAS compared with cmlc2/RFP, without significant changes in 7 dpci compared with uninjured hearts. (B) Selected enriched gene ontology terms for the 1040 genes exclusively upregulated in HRAS-induced hearts. Cancer-associated, cell cycle transition and proteasome genes are indicated. (C) Selected enriched gene ontology terms for the 937 genes exclusively downregulated in HRAS-induced hearts. Examples of tumor suppressor genes and histone modifiers are listed. (D,E) Immunostaining of regenerating and HRAS-expressing hearts reveals differential subcellular localization of HMGB1. Only in HRAS-OE hearts is this protein non-nuclear. n=4.